Extended Data Fig. 3: Collagen I and collagen III staining and quantification in aorta.
From: Fasting-mimicking diet prevents high-fat diet effect on cardiometabolic risk and lifespan
Extended Data Fig. 3 related to Fig. 4. Collagen I and collagen III staining and quantification in aorta. a) Representative image of Collagen I immunostaining in aorta. Collagen I (green), DAPI (blue). b) Immunofluorescence quantification of Collagen I expression. One-way ANOVA, p = 0.2549 ns. c) Representative image of Collagen III immunostaining in aorta. Collagen III (green), DAPI (blue). d) Immunofluorescence quantification of Collagen III expression. One-way ANOVA, p = 0.0285 *. Tukey’s multiple comparisons tests; Control vs. HFCD, p = 0.0320 *, Control vs. HFCD + FMD, p = 0.8694 ns, HFCD vs. HFCD + FMD, p = 0.0830 ns. Data are shown as mean ± SEM, n = 6 mice per group.
