Fig. 8: TRAP-based RNA sequencing identifies coordinated regulation of mitochondrial quality control.
a,b, Volcano plots of nonsignificant genes (Nonsignificant, adjusted P > 0.05) and significant (adjusted P < 0.05) tanycyte-unrelated (Rest significant) and tanycyte-related (Tanycyte) genes in NCD control/IRΔTan (a) and NCD control/HFD control (b), shown as DEGs. c, Overlap analysis of DEGs in tanycytes of both comparisons (coloured region) revealed 60 out of 74 and 28 out of 50 tanycyte-specific upregulated and downregulated genes, respectively. d, GO analysis revealed upregulated biological processes and molecular functions in tanycytes of NCD control/IRΔTan versus NCD control/HFD control. P values are FDR-adjusted using Benjamini–Hochberg correction. a,b, Significantly differentially enriched transcripts (P ≤ 0.05) are indicated in the coloured region (P values were FDR-adjusted for multiple comparisons using Wald test). Red dashed line indicates the significance level (adjusted P < 0.05). Highlighted genes were selected from upregulated GO terms: protein localization to mitochondrion (Timm50, Dnaja1, Hsp90aa1, Hspd1, Hsph1), neuropeptide receptor activity (Nmbr, Nmur2), IR binding (Irs4). c, Red dashed line indicates the log2(fold change) = ±1. e–h, Expression values of selected upregulated genes with low (e), medium (f) or high (g) expression and of selected downregulated (h) genes. n = 3/NCD control mice and NCD IRΔTan, n = 4/HFD control. CPM, counts per million reads; FDR, false discovery rate.
