Extended Data Fig. 1: Cd toxin production confers advantage in vivo and generates distinct metabolome.
From: Oxidative ornithine metabolism supports non-inflammatory C. difficile colonization
a, Experimental gnotobiotic mouse model for Cd colonization in non-inflamed conditions. Germ-free mice were tri-colonized with B. thetaiotaomicron, C. sporogenes and E. coli 12–15 days prior to infection with WT (630 or R20291) or Tox- (630 TcdA−TcdB− or R20291 TcdA−TcdB−CDT− Cd or gavaged with PBS. Mice were maintained on standard diet and no antibiotic pretreatment was used. b, WT R20291 reaches a higher relative abundance in the presence of a defined commensal community (DC 1, mean ± s.e.m., n = 5 mice/group, unpaired two-tailed t-test) than its toxin-deficient counterpart. Absolute abundance (CFU/mL feces) of the two strains did not differ (data not shown). c, R20291 toxin production differentiates the metabolic milieu of cecal contents. Principal components analysis with 95% confidence regions for mice infected with WT (n = 5 mice, red), Tox- (n = 5, purple) or uninfected controls (n = 4, yellow).
