Extended Data Fig. 2: Untargeted metabolomics demonstrates toxin-dependent changes to metabolites in gut lumen.
From: Oxidative ornithine metabolism supports non-inflammatory C. difficile colonization
a, 75 metabolites with the greatest variance across groups of mice infected with R20291 WT (n = 5 mice), Tox- (n = 5) or uninfected controls (n = 4) as described in Extended Data Fig. 1a (row-normalized log10-transformed peak areas shown). Amino acids are noted with asterisks; metabolites in (b) are highlighted in red. b, Normalized peak values for identified metabolites related to ornithine metabolism. Ornithine and its reductive end-product 5-aminovaleric acid exhibit inverse trends (group sizes are as in a, mean ± s.e.m. shown; one-way ANOVA across all named metabolites with FDR-adjusted P value (Padj) < 0.1, * denotes significance by Fisher’s LSD post hoc comparison: ornithine F = 5.5103, Padj = 0.0849; citrulline F = 20.078, Padj = 0.0029, 5-aminovaleric acid F = 5.3369, Padj = 0.0895; putrescine F = 11.315, Padj = 0.0162).
