Extended Data Fig. 4: Dietary ornithine boosts Cd abundance in the mouse gut.
From: Oxidative ornithine metabolism supports non-inflammatory C. difficile colonization
a, Ornithine supplementation (1% w/v in drinking water) to conventional mice fed an ornithine-free diet co-infected with equal amounts WT and ∆oraSE leads to higher overall Cd abundance in cecal contents (mean ± s.e.m., n = 5 mice/group; unpaired two-tailed t-test). WT Cd reaches higher abundance in feces (b, n = 5 mice/group, mean ± s.e.m.) and cecal contents (c, n = 5 mice/group, mean ± s.e.m.; unpaired two-tailed t-test) of conventional mice fed standard diet supplemented with 1% ornithine in drinking water. d, Dietary ornithine availability does not alter toxin production on a per-cell basis in mice harboring a defined consortium of bacteria infected with WT or ∆oraSE Cd fed an ornithine-free diet (1% ornithine w/v administered in drinking water, denoted by shaded blue boxes; n = 5 mice/group, mean ± s.e.m.; multiple unpaired t-tests, FDR-adjusted with two-stage linear step-up). e, Exogenous ornithine provided in drinking water (1% w/v) to conventional mice fed standard diet does not influence serum lipocalin-2 levels (mean ± s.e.m., n = 5 mice/group).
