Extended Data Fig. 10: HDAC6 inhibitors induce heat shock response.
From: Histone deacetylase 6 inhibition restores leptin sensitivity and reduces obesity
a, b, HSP70 mRNA level in iWAT explants from DIO mice 24 hr after, vehicle (n = 41) vs. TubA (n = 42, P = 1.1E-3) (a) or Vehicle (n = 4) vs. CAY10603 (n = 4, P = 3.8E-4, by two-tailed unpaired t-test for a and b) (b) treatments. c, HSP25 and HSP70 mRNA expression in 293 T cells transfected with the indicated constructs (n = 6. HSP25: GFP vs. HDAC6CI P = 1.5E-9, HDAC6 WT vs. HDAC6CI P = 2.8E-9; HSP70: GFP vs. HDAC6CI P = 3E-5, HDAC6 WT vs. HDAC6CI P = 4.5E-9, GFP vs. HDAC6 WT P = 3.1E-5). d, TSC2 +/+ and TSC−/− mouse embryonic fibroblasts (MEFs) were treated with vehicle (DMSO) or celastrol for 24 hr. Cell lysates were analyzed by immunoblots. The results were confirmed in three independent experiemnts e, p-PERK and total PERK protein levels in liver homogenates from wild-type DIO mice treated with vehicle or celastrol. Th experiment was conducted in two independent cohorts of mice with similar outcomes. Data are represented as mean ± s.e.m.
