Extended Data Fig. 3: The effect of mPGES-2 on β-cell function under physiological conditions.
From: mPGES-2 blockade antagonizes β-cell senescence to ameliorate diabetes by acting on NR4A1
a, qRT–PCR analysis of Mafa, Slc2a2, Nkx6-1 and Pdx1 expression in WT and KO islets at 32 weeks (n = 5 per group). b, Representative IF for Glut2 (green) and insulin (red) in islets of WT and KO mice fed an NCD at 16, 52 weeks and an HFD at 52 weeks; scale bars = 20 μm. c, Statistical quantification of IF for Glut2 (n = 4 per group). d, H&E staining of pancreas in db/db Ptges2+/+ and db/db Ptges2−/− mice (n = 4 per group); scale bars = 20 μm. e, Body weights of db/db Ptges2+/+ and db/db Ptges2−/− mice at different weeks (n = 10 per group). f, Representative IF and quantification for Glut2 (green) and insulin (red) in islets of db/db Ptges2+/+ and db/db Ptges2−/− mice; scale bars = 20 μm (n = 5 per group). H&E staining and IF were repeated more than twice, independently and similar results were obtained. Data are expressed as mean ± SEM. Statistical significance was assessed using a two-tailed unpaired Student’s t-test. Exact P values are indicated.
