Extended Data Fig. 5: Assessment of mPGES-2 function in β-cells.
From: mPGES-2 blockade antagonizes β-cell senescence to ameliorate diabetes by acting on NR4A1
a, PGE2 levels were determined in tissues of liver, kidney, or brown fat in mPGES-2 WT and mPGES-2 KO mice (n = 5 per group). b, Islets were isolated from mPGES-2 WT and KO mice to assess the expression levels of different PGE2 synthases. Levels of Ptges, Ptges2 and Ptges3 mRNA expression (n = 4 WT, n = 5 KO). c, Immunoblotting and quantification of mPGES-2 in INS-1 cells (n = 4). d, NR4A1 expression induced by 10 μM 8-Br-cAMP at different times (n = 5), the samples derive from the same experiment and that gels/blots were processed in parallel. e, The expression of p21 in NR4A1-overexpressing INS-1 cells (n = 6). f, The expression of p21 and p16 in INS-1 cells with mPGES-2 overexpression or NR4A1 overexpression or dual overexpression (n = 4). g, The effect of mPGES-2 knockdown on NR4A1 signaling pathway, senescence markers and quantification of western blotting in INS-1 cells (n = 4). NC, negative control; KD, knockdown. h, The influence of mPGES-2 knockdown on insulin secretion under low glucose or high glucose (n = 3). Data are expressed as mean ± SEM. Statistical significance was assessed using one-way ANOVA with Tukey’s test (d and f) and a two-tailed unpaired Student’s t-test (a, b, c, e, g and h). Exact P values are indicated.
