Extended Data Fig. 6: Effects of SZ0232 on PGE2 synthase inhibitory rates and insulin secretion in vitro.
From: mPGES-2 blockade antagonizes β-cell senescence to ameliorate diabetes by acting on NR4A1
a, Cell viability of INS-1 cells at the indicated concentrations of SZ0232 (n = 6). b, Inhibition of COX-2 enzyme activity by SZ0232 (n = 3). c, Inhibition of mPGES-1 enzyme activity by SZ0232 (n = 3). d, Effect of SZ0232 on insulin secretion in islets isolated from WT mice at the indicated weeks (n = 3). e, Effects of SZ0232 on insulin secretion in islets isolated from mPGES-2 WT and KO mice under low glucose conditions (n = 3). f, Effects of SZ0232 on insulin secretion in islets isolated from mPGES-2 WT and KO mice under high glucose conditions (n = 3). g, Effects of SZ0232 on insulin secretion in islets isolated from β-WT and β-KO mice under high glucose conditions (n = 4 for control group, n = 3 for SZ0232 group). h, Representative images and quantification of β-Gal staining of INS-1 cells with or without mPGES-2 overexpression or OE cells treated with SZ0232 (n = 4); scale bars = 20 μm. β-Gal staining was repeated more than twice, independently, with similar results. Data are expressed as mean ± SEM. Statistical significance was assessed using a two-tailed unpaired Student’s t-test (d, e, f and g) and one-way ANOVA with Tukey’s test (h). Exact P values are indicated.
