Extended Data Fig. 6: Low endothelial Pten expression correlates with improved glucose systemic metabolism in mice.
(a) Tissue weight in control and PteniΔEC mice exposed to HFD for 10 weeks (control n = 9, PteniΔEC n = 4). (b) Insulin tolerance test (ITT) performed in control and PteniΔEC mice fed with HFD for 10 weeks (control n = 13, PteniΔEC n = 10). Bars to the right show AUC quantification of blood glucose monitoring during ITT (control n = 13, PteniΔEC n = 10). (c) Basal insulin levels and (d) HOMA-IR index in control and PteniΔEC mice fed with HFD for 10 weeks (control n = 6, PteniΔEC n = 4). (e) Masson trichrome fibrosis staining in eWAT and iWAT sections from control and PteniΔEC mice exposed to HFD for 10 weeks. (f) Gene expression analysis of fibrosis markers in whole tissue extracts from eWAT (control n = 7, PteniΔEC n = 4) from control and PteniΔEC mice exposed to HFD for 10 weeks. (g) Tissue weight in control and PteniΔEC mice exposed to HFD for 34 weeks (control n = 9, PteniΔEC n = 8). (h) ITT performed in control and PteniΔEC mice fed with HFD for 30 weeks (control n = 10, PteniΔEC n = 9). Bars to the right show AUC quantification of blood glucose monitoring during ITT. (i) Basal insulin levels and (j) HOMA-IR index in control and PteniΔEC mice fed with HFD for 34 weeks (control n = 9, PteniΔEC n = 8). (k) Masson trichrome fibrosis staining in eWAT and iWAT sections from control and PteniΔEC mice exposed to HFD for 34 weeks. Data represent mean ± SEM (error bars). Samples represent biological replicates. Statistical analysis was performed by the two-sided Mann-Whitney test (Extended Data Fig. 6a, c, d, f, i, j) and 2-way ANOVA with Bonferroni correction (Extended Data Fig. 6b, h).
