Fig. 6: Rewiring of PPP and changes in redox status in murine neutrophils activated in vivo and in classically activated macrophages.
a, Relative NADPH/NADP+ redox ratio and PPP metabolite levels in neutrophils isolated from either bone marrow (BM) or peritoneal cavity (PC) of mice following peritoneal injection of MSU. Bar graphs show mean ± s.d., dots represent biological replicates; n = 5 mice. Numbers of effective measurements of specific compounds per condition (indicated by individual dots) vary due to insufficient number of neutrophils isolated from bone marrow in one of the mice for the complete LC–MS analyses. P values were determined by two-sided, unpaired t-test. b, Schematic showing the connection between PPP and NOS2 in macrophages. c, Metabolite abundance in WT or NOS2 KO bone marrow-derived macrophages stimulated with LPS and IFN-ɣ. Mean ± s.d., n = 3 Technical replicates. Results were repeated in two independent experiments. d, Metabolite abundance in WT bone marrow-derived macrophages stimulated with LPS and IFN-γ for 48 h with or without treatment by 6-AN (1 mM). Mean ± s.d., n = 3 technical replicates.
