Extended Data Fig. 1: Oxidative burst relies on oxidative PPP, and the rewiring of PPP is coupled to the oxidative burst (Related to Fig. 1).
a. The oxidative burst induced by PMA (100 nM) is inhibited by varying concentrations of 6-AN or G6PDi. (Mean ± SD, N = 6 technical replicates). b Relative levels of PPP intermediates 6PG, ribulose-5-phosphate, S7P, and glycolytic intermediates: FBP, DHAP, and lactate 30 minutes after 100 nM PMA stimulation, with or without the treatment of G6PDi (50 µM) or DPI (10 µM). Measurements were made in neutrophils isolated from 5 donors, as indicated by color codes. (Neutrophil yield from every donor was not always high enough to cover all three conditions, so N = 5 PMA, N = 4 PMA + DPI, N = 3 PMA + G6PDi). Relative level is normalized to neutrophils stimulated with 100 nM PMA without inhibitor from the same donor. Bar graph indicates mean value of two technical replicates, as indicated by dots. Statistical significance was determined by paired, two-tailed t-test across biological replicates (mean values of technical replicates per donor), with p-values indicated in the figure c. Dose-dependent changes in the levels of PPP intermediates in human peripheral blood neutrophils stimulated with various doses of zymosan with or without DPI (10 µM). Bar graphs show mean value of technical duplicates indicated by dots. d. Oxidative burst induced by various doses of zymosan A, Mean ± SD, N = 6 technical replicates.
