Extended Data Fig. 6: Effects of itaconate and mesaconate are independent of GSH.
From: Mesaconate is synthesized from itaconate and exerts immunomodulatory effects in macrophages
a. Cytokine secretion of BMDMs from wildtype mice pre-treated with 10 mM itaconate or mesaconate, or 0.25 mM DMI, in the presence or absence of 1 mM cellular permeable GSH (EtGSH) for 4 h prior to LPS stimulation for 21 h. b,c. Cytokine secretion (b), glucose uptake and lactate secretion (c) of Gclc-deficient (Gclc-KO) BMDMs pre-treated with 10 mM itaconate or mesaconate for 4 h prior to LPS stimulation for 21 h. Data are shown as: a. mean ± SEM calculated from n = 4 mice; b,c. mean with individual mouse data pooled from n = 6 (b) or 3 (c) mice, and conditions from individual mouse are connected with a line. P values were calculated by multiple unpaired t-test (a) or paired t -test (b,c, paired by each mouse) and overlayed on respective comparisons.
