Extended Data Fig. 5: Dependency of DMI and 4-OI on NRF2 and ATF3 for their anti-inflammatory effects in mouse BMDMs.
From: Mesaconate is synthesized from itaconate and exerts immunomodulatory effects in macrophages
Cytokine secretion of BMDMs from wildtype (WT) and Nrf2-KO (a) or Atf3-KO (b) mice pre-treated with 0.25 mM DMI or 0.125 mM 4-OI for 4 h prior to LPS stimulation for 21 h. Data are shown as mean ± SEM calculated from n = 5 (a) or 3 (b) mice. P values were calculated by multiple unpaired t-test and overlayed on respective comparisons.
