Extended Data Fig. 4: Cold-induced resolution of inflammation precedes changes in lipid accumulation in the liver of DIO mice, related to Fig. 2.
From: Brown adipose tissue-derived MaR2 contributes to cold-induced resolution of inflammation
(a-c) C57BL6/J male mice were fed with normal chow (NC) or high-fat (HF) diet at room temperature for 14 weeks. The mice were then exposed to thermoneutral temperature (30 °C, TN) or cold (5 °C) for 7 days. (a) Relative mRNA expression of lipogenesis-related genes in the liver (n = 5 biologically independent animals per group, from 1 independent experiment). (b) Liver TG levels (n = 5 biologically independent animals per group, from 1 independent experiment). (c) Representative images of hematoxylin and eosin (H&E) staining of liver sections from NC-TN, HF-TN and HF-cold groups (Scale bar=100 μm). (d-g) C57BL6/J male mice were fed with normal chow (NC) or high-fat (HF) diet at room temperature for 14 weeks. The mice were then put in thermoneutral temperature (30 °C, TN) or cold (5 °C) for 2 days. (d) Relative mRNA expression of lipogenesis-related genes in the liver (n = 5 biologically independent animals for NC-TN and NC-cold and HF-cold, n = 4 for HF-TN, from 1 independent experiment). (e) Liver TG levels (n = 5 biologically independent animals per group, from 1 independent experiment). (f) Representative images of hematoxylin and eosin (H&E) staining of liver sections from HF-TN and HF-cold groups (Scale bar=100 μm). (g) Relative mRNA expression of proinflammatory, NLRP3 inflammasome-related- and fibrosis-related genes in the liver (n = 5 biologically independent animals for NC-TN, NC-cold and HF-cold, n = 4 for HF-TN, Casp1 NC-cold, Casp1 HF-cold, Il18 HF-cold, Tlr4 HF-cold, from 1 independent experiment). One-Way ANOVA followed by a Tukey’s post hoc test. Data are presented as mean ± SEM.
