Extended Data Fig. 6: Identification of maresin pathway products in ingWAT of obese mice exposed to cold and in plasma of mice with BAT removal, related to Fig. 4 and Fig. 5.
From: Brown adipose tissue-derived MaR2 contributes to cold-induced resolution of inflammation
(a) Relative expression of Ucp1 mRNA in ingWAT of HF-fed (14 weeks) C57BL6/J mice exposed to TN or cold for 7 days (n = 5 biologically independent animals per group, from 1 independent experiment). (b, c) Quantification of 14-HDHA, MaR1, and MaR2 in ingWAT and normalized to tissue weight (n = 6 biologically independent animals for HF-TN, n = 5 for HF-cold, from 1 independent experiment; b) or protein concentration (n = 6 biologically independent animals for HF-TN, n = 5 for HF-cold, from 1 independent experiment; c). (d, e) MRM peaks (d) and MS/MS spectra with diagnostic ions labeled (e) of authentic MaR2 standard and of two MaR2 isomers (denoted isomer I and II) identified in a selected sample of plasma of HF-fed mice following BAT removal. ND: not detected. Two-tailed Unpaired Student’s t-tests. Data are presented as mean ± SEM.
