Extended Data Fig. 8: MaR2 resolves inflammation systemically and in the liver of DIO mice, related to Fig. 8.
From: Brown adipose tissue-derived MaR2 contributes to cold-induced resolution of inflammation
(a) Schematic of the experimental design of MaR2 treatment. C57BL6/J male mice were fed with a high-fat (HF) diet at room temperature for 14 weeks. Then the mice were administered vehicle or MaR2 (5 μg/kg/day; daily i.p.) for 28 days. (b) Body weight at day 0 and day 28 post-treatment (n = 5 biologically independent animals per group, from 1 independent experiment). (c) Plasma TNF-α levels post-treatment (n = 5 biologically independent animals per group, from 1 independent experiment). (d) Relative mRNA expression of proinflammatory, NLRP3-inflammasome-related- and fibrosis-related genes in the liver post-treatment (n = 5 biologically independent animals per group, from 1 independent experiment). (e) Schematic of the experimental design of MaR2 treatment. C57BL6/J male mice were fed with a high-fat (HF) diet at room temperature for 16 weeks. Then, the mice were administered vehicle or MaR2 (10 μg/kg/day; daily i.p.) for 26 days. (f) Body weight at day 0 and day 25 post-treatment, and the body weight change from baseline (n = 6 biologically independent animals per group, from 1 independent experiment). (g) Total caloric intake (n = 6 biologically independent animals per group, from 1 independent experiment). (h, i) Liver weight and TG levels in liver (n = 6 biologically independent animals per group, from 1 independent experiment). (j) Relative mRNA expression of lipogenesis-related genes in liver (n = 6 biologically independent animals per group, from 1 independent experiment). (k) Representative liver H&E staining from each group (Scale bar=100 μm). (l) Plasma levels of ALT in mice treated with vehicle or MaR2 (n = 6 biologically independent animals per group, from 1 independent experiment). (m, n) Relative mRNA expression of inflammation and NLRP3-inflammasome-related genes in the BAT (n = 6 biologically independent animals per group, from 1 independent experiment, m) and epiWAT (n = 5 for Vehicle, except Casp1 n = 4, n = 6 for MaR2, from 1 independent experiment, n) of mice treated with vehicle or MaR2. Two-tailed Unpaired Student’s t-tests. Data are presented as mean ± SEM.
