Extended Data Fig. 9: Effects of gene FBP1, RPE, and TAL1 on cell growth and FFA production in faa1Δ strain in methanol medium.
From: Rescuing yeast from cell death enables overproduction of fatty acids from sole methanol
a, Genotype of engineered strains. According to transcriptional analysis, most genes were downregulated except for genes such as FBP1, RPE, and TAL1. For these three genes, coupled with disrupted lpl1 and izh3, were tested in faa1Δ strain. Engineered strains was cultivated in minimal methanol medium to detect the cell growth curve (b) and FFA production (c). Genes FBP1, RPE, and TAL1 failed to restore cell growth and FFA production without the double deletion of genes LPL1 and IZH3. Data are presented as mean ± s.e.m. (n = 3 biologically independent samples).
