Extended Data Fig. 10: Engineering evolved strain to improve FFA production from methanol by manipulating genes related to supply of acetyl-CoA and NADPH.
From: Rescuing yeast from cell death enables overproduction of fatty acids from sole methanol
a, The final biomass (OD600) of engineered strains. b, The final FFA titer of engineered strains. Evolved strain HpFAM02 (HpFAM01, ura3Δ, leu1.1:: HpLEU2) was used as the control strain. To increase the acetyl-CoA supply, a modified β-oxidation (pex10Δ), citrate lysis (MmACL), and PDH complex mutant from Escherichia coli were introduced. To increase the supply of NADPH, an enhanced gluconeogenesis (FBP1) and oxidative stage of PPP (ZWF1) was achieved. The introduction of PDHm did not cause any significant difference in growth and FFA production. Overexpression of MmACL and FBP1-ZWF1 increased the fatty acid titers by 10% and 30%, respectively. Data are presented as mean ± s.e.m. (n = 3 biologically independent samples). c, Proportion of unsaturated FFAs increased in a ScIDP2-expressing strain. d, Intracellular NADPH/NADP+ in strains with, without ScIDP2 overexpression. e, The expression level of genes that encode fatty acid desaturase (Δ9, Δ12, Δ15) in strains with, without ScIDP2 overexpression. Data are presented as mean ± s.e.m. (n = 3 biologically independent samples). f, Electron transfer in the process of desaturation of FFAs.
