Extended Data Fig. 1: Effects of extra nutrition, glucose and xylose on cell growth of faa1Δ strain in methanol medium.
From: Rescuing yeast from cell death enables overproduction of fatty acids from sole methanol
a, Fermentations were conducted in complex medium (YPM) containing 20 g/L peptone, 10 g/L yeast extract and 10 g/L methanol. b-d, faa1Δ cells were cultivated in Delft minimal medium containing various ratios of glucose and methanol that were equal to the molar amount of 10 g/L methanol. e-i, Xylose promoted cell growth and methanol assimilation of faa1Δ strain due to enhanced Xu5P supply. faa1Δ cells were cultivated in minimal medium containing substrates that were equal amount of carbon atoms to 10 g/L methanol (M), including sole xylose (Xyl) and different ratios of mixed methanol and xylose of 7:3 (7 M:3X), 8:2 (8 M:2X), 9:1 (9 M:1X). In particular, the sole xylose of 3X, 2X and 1X were used as controls to illustrate the utilization of methanol for cell growth and FFA production in the presence of xylose. Cells cultivated in sole methanol (YPM) and mixed methanol and xylose, were collected to extract intracellular metabolites and Xu5P was determined by LC–MS/MS. Data are presented as mean ± s.e.m. (n = 3 biologically independent samples).
