Extended Data Fig. 2: Enhancing the supply of xylulose 5-phosphate and NADPH in faa1Δ strain.
From: Rescuing yeast from cell death enables overproduction of fatty acids from sole methanol
a, Scheme of the rational design for the enhancement of Xu5P and NADPH, by enhancing gluconeogenesis, the pentose phosphate pathway and IDP2. b, Cell growth of strains with engineered NADPH related genes. Data are presented as mean ± s.e.m. (2 biologically independent samples were adopted).c, Overexpressing the Xu5P supply related genes TKL1, TALs, RPE, RKIs slightly increased the final amount of cell biomass but failed to fully restore growth in methanol medium. Data are presented as mean ± s.e.m. (3 biologically independent samples were adopted). For all experiments, strains were pre-cultured in YPD for 16~18 h and then washed twice with Delft medium and subsequently transferred into Delft medium with the initial OD600 of 0.2. Strains were cultivated at 37oC, 220 rpm for biomass analysis.
