Extended Data Fig. 2: Tissue specific transcriptional responses upon Tyr restriction.
a, Number of differentially expressed genes (Benjamini–Hochberg adjusted p-value < 0.01 after Wald test) in the gut or the fat body of third instar larvae during Tyr restriction. The numbers of either up- (log2 fold change > 0.59) or down- (log2 fold change < −0.59) regulated genes are shown. b, Quantitative RT-PCR of 4E-BP in the gut of third instar larvae during Tyr restriction. n = 9. P-value is determined by unpaired two-tailed Student’s t-test. c,d, Representative images of the fat body (c) or the other organs (d) of the ATF4 reporter 4E-BPintron-dsRed with or without Tyr restriction for 28 hours. Scale bars, 1 mm for the gut, 100 μm for the fat body, the salivary gland and the carcass. Ctrl indicates a complete holidic medium. For the graph, Mean and SEM were shown. Data points indicate biological replicates. ns, not significant. The experiments were repeated independently at least twice with similar results (c, d).
