Extended Data Fig. 3: Phenotypic and metabolic analysis upon amino acid restrictions.
a, Fecundity of female flies upon AA restriction for four days. n = 8. Any EAA restrictions significantly (p < 0.0001) suppressed egg laying, while any NEAA did not. b, Body weight of third instar larvae upon Tyr or Leu restriction for 24 hours. n = 28 (Tyr-), 37 (Leu1/2), 30 (Leu1/4), 31 (Leu-). c, Whole body Tyr levels in female flies upon Tyr restriction for four days. n = 6. d,e, Whole body NEAA (d) or EAA (e) levels in adult male flies upon each NEAA (d) or EAA (e) restriction for four days. n = 6. f, Whole body Tyr metabolite levels in the third instar larvae upon Tyr restriction. n = 4. g, Whole body amount of Arg levels in the third instar larvae upon Arg restriction for 8 h. n = 6. Ctrl indicates a complete holidic medium. For all graphs, Mean and SEM were shown. Data points indicate biological replicates. P-values are determined by one-way ANOVA with Dunnett’s multiple comparison test (a), one-way ANOVA with Holm-Ĺ Ădák’s multiple comparison test (b) and unpaired two-tailed Student’s t-test (c-g). Asterisks indicate *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001, ns, not significant.
