Extended Data Fig. 5: Suppression of mTORC1 activity upon Tyr or Leu restriction.
a, b, A representative image (a) and quantification (b) of western blot analysis of the fat body using anti-phospho-S6 antibody upon Tyr or Leu restriction (1/2, 1/4 and complete depletion) for 8 h. Ctrl indicates a complete holidic medium. Anti-α+β tubulin was used for loading control. n = 3. P-value is determined by one-way ANOVA with Dunnett’s multiple comparison test. For the graph, Mean and SEM with all data points of biological replicates were shown. Asterisks indicate ****p < 0.0001.
