Extended Data Fig. 1: Internal AA levels during protein restriction.

a-d, Internal Tyr and Phe levels in the fat body (a, b) or hemolymph (c, d) of third instar larvae fed with a low-protein diet supplemented with or without 5 mM Tyr for 8 h. n = 4. e,f, Internal AA levels in the fat body (e) or hemolymph (f) of third instar larvae fed with a low-protein diet for 8 h. n = 4. For all graphs, Mean and SEM with all data points of biological replicates were shown. P-values are determined by one-way ANOVA with Holm-Ĺ Ădák’s multiple comparison test (a-d) and unpaired two-tailed Student’s t-test (e,f). Asterisks indicate *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001, ns, not significant.