Extended Data Fig. 6: METTL5 knockdown impairs fatty acid β-oxidation.
From: N6-methyladenosine (m6A) in 18S rRNA promotes fatty acid metabolism and oncogenic transformation
a, b, OCRs of control and METTL5-knockdown HCC cells treated with BSA, palmitate-BSA (PA) and control medium or etomoxir (ETO). After sequential injection of ETO (4 μM) or control medium, oligomycin (Oligo, 1.5 μM), FCCP (1 μM) and rotenone/antimycin (R/A, 0.5 μM), a Seahorse Extracellular Flux (XF) Analyzer was used to perform this assay. The data are shown as the mean ± SD. n = 6 samples. c, d, Basal respiration of control and METTL5-knockdown HCC cells. n = 6 samples. e, f, Maximal respiration of control and METTL5-knockdown HCC cells. n = 6 samples. g, Acute response (decrease in basal OCR after incubation with etomoxir) of control and METTL5-knockdown HCC cells. n = 6 samples. In the histograms, the data are shown as the mean ± SD with P-values labeled on individual panels. P values were indicated by two-tailed unpaired Student’s t test for (c-g) in this figure. (a-g) the unit for n is “samples”.
