Extended Data Fig. 8: Restoring ACSL4 expression in METTL5-knockout cells promotes fatty acid β-oxidation.
From: N6-methyladenosine (m6A) in 18S rRNA promotes fatty acid metabolism and oncogenic transformation
a-d, Intracellular free fatty acids (a), triglycerides (b), cholesterol (c) and lipid droplets (d, e) in METTL5-knockout HepG2 and Huh7 cells with or without ACSL4 overexpression. n = 3 replicates. Scale bar, 200 μm. f, g, OCR of METTL5-knockout HepG2 and Huh7 cells with or without ACSL4 overexpression. The data are shown as the mean ± SD. n = 6 samples. h-m, Basal respiration (h, i), maximal respiration (j, k) and acute responses (l, m) of control and METTL5-knockout HepG2 and Huh7 cells with or without ACSL4 overexpression. n = 6 samples. (a-e) the unit for n is “replicates”. (f-m) the unit for n is “samples”.
