Extended Data Fig. 3: ORM2 overexpression attenuates hepatic steatosis and hyperlipidemia in db/db mice and HFD-induced obese mice.
From: Orosomucoid 2 maintains hepatic lipid homeostasis through suppression of de novo lipogenesis
Related to Fig. 4. a-j, 8-week-old db/db male mice were administered with adenovirus containing ORM2 (Ad-ORM2) or GFP (Ad-GFP) through tail vein injection. a, b, Protein ORM2 levels were determined by western blots using the livers (a, top) and plasma (b, bottom) from two groups of mice. n = 6 biologically independent mice per condition. c, Relative mRNA expression levels of ORM1, ORM2 and ORM3 in the liver. n = 6 biologically independent mice per condition. d-i, Body weight (d), average daily food intake for 3 consecutive days (e), liver/body weight ratio (f), hepatic TG (g), plasma TG (h) and TC (i) levels from two groups of mice. (d-i) Ad-GFP: n = 8 biologically independent mice. Ad-ORM2: n = 9 biologically independent mice. j, H&E and Oil Red O staining of liver sections. Scale bar, 50 μm. Representative result from three biologically independent mice. k-q, C57BL/6 J male mice were fed a normal diet or HFD for 12 weeks and then administered with Ad-GFP or Ad-ORM2 for 12 days. k, Protein levels of ORM2 in the livers from three groups of mice. n = 4 biologically independent mice per condition. Liver/body weight ratio (l), hepatic TG (m), plasma TG (n) and TC (p) levels and body weight (q) from three groups of mice. ND + Ad-GFP: n = 4 biologically independent mice. HFD + Ad-GFP: n = 6 biologically independent mice. HFD + Ad-ORM2: n = 7 biologically independent mice. o, Macroscopic appearance of liver, H&E and Oil Red O staining of liver sections. Scale bar, 50 μm. Representative result from three biologically independent mice. Data are shown as mean ± SEM (c-i, l-n, p, q). P values are determined by unpaired two-tailed Student’s t-test (c-i) and one-way ANOVA followed by Dunnett’s multiple comparisons test (l-n, p, q).
