Extended Data Fig. 6: Effects of adipocyte IRE1α abrogation upon iWAT transcriptome and BAT thermogenic program.
From: Adipocyte IRE1α promotes PGC1α mRNA decay and restrains adaptive thermogenesis
a-f, Temperature-dependent effects of adipocyte IRE1α abrogation upon iWAT transcriptome. Bulk RNA-seq analysis of iWAT RNAs was performed for Ern1fl/fl and Ern1AKO mice housed at 30oC or following 3 days of cold exposure at 8oC as described in Fig. 4a (n = 3 mice per group). (a) Volcano plot highlighting significantly upregulated and downregulated genes (fold change >1.5 and ANOVA p-value <0.05) in Ern1AKO iWAT relative to Ern1fl/fl iWAT when housed at 30oC. (b,c) Gene ontology (GO) biological process (BP) analysis of downregulated (b) and upregulated genes (c) resulting from IRE1α abrogation in mice at 30oC. (d) Volcano plot highlighting significantly upregulated and downregulated genes (fold change >1.5 and ANOVA p-value <0.05) in Ern1AKO iWAT relative to Ern1fl/fl iWAT after cold exposure at 8oC. (e,f) GO biological process analysis of downregulated (e) and upregulated genes (f) resulting from IRE1α abrogation after cold exposure. g-j, Adipocyte IRE1α deficiency does not significantly affect the adaptive thermogenic gene program in BAT. (g,h) qRT-PCR analysis of Xbp1 mRNA splicing (g) and mRNA abundance of the indicated thermogenic genes (h) in BAT of mice following 3 days of cold exposure or at thermoneutrality (n = 5 per genotype at 30oC; n = 10 per genotype at 8oC). (i,j) qRT-PCR analysis of Xbp1 mRNA splicing (i) and mRNA abundance of the indicated thermogenic genes (j) in BAT of mice following 3 days of daily saline or CL316,243 injection (saline control, n = 6 each genotype; CL316,243 treatment, n = 9 for each genotype). Data are presented as mean ± s.e.m.; *P < 0.05, **P < 0.01 by two-way ANOVA (g,i).
