Extended Data Fig. 4: EphB4 expression pattern.
From: Insulin induces insulin receptor degradation in the liver through EphB4
a, b, EphB4 mRNA (a, n = 3 mice) and protein (b, n = 3 mice) levels in the livers of HFD-fed mice. EPHB4 proteins were detected in the same samples used to detect Actin levels in Extended Data Fig. 1d. c, d, EPHB4 mRNA level in HepG2 cells (c, n = 5 biologically independent cells) and primary hepatocytes (d, n = 4 biologically independent cells) stimulated with insulin (100 nM). For statistical analysis in a-d, comparisons were versus related controls, using two-sided Student’s t-test. Values are presented as the mean ± SEM. NC, normal chow; HFD, high fat diet.
