Extended Data Fig. 9: A pharmacological EphB4 inhibitor improved metabolism in mice.
From: Insulin induces insulin receptor degradation in the liver through EphB4
a, b, c, Effect of LCA on the insulin-stimulated increase in p-AKT levels in HepG2 cells (a, b, n = 3 biologically independent cell samples) and primary hepatocytes (c). d, e, Gluconeogenesis in primary hepatocytes from HFD-fed mice (d, n = 4 biologically independent cell samples) or db/db mice (e, n = 3 biologically independent cell samples) treated with LCA. f, Body weight of db/db mice grouped for drug administration (age: 8 weeks, n = 10 mice). g, Fasting plasma glucose level in db/db mice grouped for drug administration (n = 10 mice). h, Percent decrease in glucose levels after the insulin injection and drug administration (n = 10 mice). i, GTT of LCA-treated db/db mice (n = 9 mice). j, ITT of LCA-treated db/db mice (n = 9 mice). k, Serum TG levels in NVP-BHG712- or LCA-treated db/db mice (n = 9 mice). l-n, InsR protein level (n = 7 mice) and p-Akt levels (n = 3 mice) in the livers of LCA-treated db/db mice. For statistical analysis in b, d-e, i-k and m-n, comparisons were versus related controls, using two-sided Student’s t-test. For statistical analysis in f-h, comparisons were made for the three groups, using one-way ANOVA. p values in i denoted by asterisks (from left to right): p = 0.0069, p = 0.047, p = 0.0032, p = 0.0076, p = 0.0040. p values in j denoted by asterisks (from left to right): p = 0.022, p = 0.047, p = 0.042. Values are expressed as the mean ± SEM.
