Extended Data Fig. 4: Lonidamine and 3-BP stimulate the migration of microglia and BV2 cells.
(a) Primary microglial cells were plated onto transwell chamber inserts. Following 24 h incubation with solvent, cells were treated with Lonidamine (48 h, 300 μM) or 3-BP (48 h, 30 μM) in the present or absent of Aβ (24 h, 100 nM) as indicated in the figure. The cells were then stained with hematoxylin and eosin, and counted under Nikon inverted microscope. Scale bar, 20 µm. (b) Quantitation of the number of migrated cells, n = 3 independent experiments. (c) BV2 were plated onto transwell chamber inserts. Following 24 h incubation with solvent, cells were treated with Lonidamine (48 h, 300 μM) or 3-BP (48 h, 30 μM) in the present or absent of Aβ (24 h, 100 nM) as indicated in the figure. The cells were then stained with hematoxylin and eosin, and counted under Nikon inverted microscope. Scale bar, 20 µm. (d) Quantitation of the number of migrated BV2 cells, n = 3 independent experiments. (e) f/f or cKO primary microglia in the present or absent of Aβ (24 h, 100 nM) were plated onto transwell chamber inserts. Following 24 h incubation, the cells were then stained with hematoxylin and eosin, and counted under Nikon inverted microscope. Scale bar, 20 µm. (f) Quantitation of the number of migrated primary microglia, n = 3 independent experiments. Data represent mean ± SEM, n.s.: not significant. When compared with control group, *p < 0.05, **p < 0.01, ***p < 0.001. When compared with Aβ group, #p < 0.05, ##p < 0.01, ###p < 0.001, one-way ANOVA with Tukey’s post hoc analysis.
