Extended Data Fig. 8: HK2 inhibition increases LPL expression in microglia of AD mice brain, and LPL inhibitor blocks the beneficial effects of 3-BP/Lonidamine on microglia.
(a-c) Western blot and Real-time PCR analysis of LPL expression in cortex samples from 9-month-old 5×FAD mice and aged control WT mice treated with HK2 inhibitors or control vehicle. Immunoblots were probed with antibodies against the indicated proteins. Quantification of protein levels are showed in (b), n = 3 mice. Quantification of Lpl mRNA levels were showed in (c), n = 6 mice. (d, e) Immunofluorescence staining of LPL (red) with Iba1(green) or HK2(green) in cortex from 9-month-old 5×FAD mice treated with HK2 inhibitors. Representative confocal images are shown on panel (e), Scale bar:20 μm and 5 μm. Quantitation of LPL fluorescence intensity are showed in (e), n = 6 slices from 3 mice. Data represent mean ± SEM. When compared with control group, *p < 0.05, **p < 0.01, ***p < 0.001. When compared with Aβ group, #p < 0.05, ##p < 0.01, ###p < 0.001, one-way ANOVA with Tukey’s post hoc analysis.
