Extended Data Fig. 9: The inhibitions of PFKFB3, PKM2, and PDH did not promote phagocytosis and ATP production in microglia. | Nature Metabolism

Extended Data Fig. 9: The inhibitions of PFKFB3, PKM2, and PDH did not promote phagocytosis and ATP production in microglia.

From: Microglial hexokinase 2 deficiency increases ATP generation through lipid metabolism leading to β-amyloid clearance

Extended Data Fig. 9

(a) Schematic diagram of inhibitors target enzymes in glycolysis pathway. (b) The ATP production of microglia treated with these inhibitors were measured, n = 3 independent experiments. (c) The ATP production of microglia treated with PFK158 (PFK inhibitor) in different dose and time quantum, n = 3 independent experiments. (d) RT-PCR was performed to test the effect of siRNA-induced mRNA Knockdown of PFKFB3 in primary microglia, n = 3 independent experiments. (e) The ATP production of microglia treated with PFK158 (PFK siRNA). n = 3 independent experiments. Data represent mean ± SEM. When compared with control group, *p < 0.05, **p < 0.01, ***p < 0.001. When compared with Aβ group, #p < 0.05, ##p < 0.01, ###p < 0.001, one-way ANOVA with Tukey’s post hoc analysis.

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