Extended Data Fig. 6: Cox7a2l is induced upon exercise specifically in the skeletal muscle.
From: COX7A2L genetic variants determine cardiorespiratory fitness in mice and human
a. Western blot analysis of Cox7a2l protein expression at rest or after 5 weeks of wheel running exercise in liver. b. BN-PAGE immunoblot with total OxPhos immunostaining (left) and Cox7a2l immunostaining (right) in isolated mitochondria from liver at rest or after 5 weeks of wheel running exercise. c. Cox7a2l relative mRNA expression in the quadriceps of DBA/2 J mice in basal conditions (sedentary) or after 2 and 4 weeks of exercise training (n = 5). d. Mitochondrial to nuclear DNA ratio measured in liver at rest or after 5 weeks of wheel running exercise. e. mRNA expression of the indicated genes measured by qRT–PCR in gastrocnemius muscle at rest or after 5 weeks of wheel running exercise. In c and e data are represented as mean ± SEM. In d data are represented as box and whiskers. Box plots center represents the median, lower and upper hinges represent the first and third quartile (25th and 75th percentiles). The whiskers extend to the largest and smallest value. Statistical analysis for c: one-way ANOVA and Dunnett’s multiple comparisons test, statistical analysis for d and e: two-way ANOVA and Sidak’s multiple comparison test.
