Fig. 3: COX7A2L lead cis-eQTL affects cellular respiration.
From: COX7A2L genetic variants determine cardiorespiratory fitness in mice and human
a, COX7A2L mRNA expression in human myoblasts after 0, 1, 4, 7 or 10 d of differentiation (REF, n = 3; ALT, n = 4). b, BN–PAGE immunoblot with UQCRC2 immunostaining (left panels) in isolated mitochondria from differentiated human myotubes cultured in glucose-free medium for 48 h. Coomassie stained gel and complex II (SDHA) immunostaining are shown on the right as loading controls. Each sample is an independent biological replicate. The experiment was repeated twice with similar results. c, Oxygen consumption rate in differentiated human myotubes measured during a mitochondrial stress test using a Seahorse flux analyzer. Cells were differentiated and then grown in glucose-free medium for 48 h. O, oligomycin; F, FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone); R/A, rotenone/antimycin (REF, n = 3; ALT n = 4). d, Average basal, ATP-linked and maximal respiration calculated from c. OCR, oxygen consumption rate. e, Western blot analysis of differentiated human myotube lines cultured for 48 h in glucose-free medium. f, Western blot quantification of e (REF, n = 3 independent biological replicates; ALT, n = 4 independent biological replicates). Data are represented as mean ± s.e.m. Each dot and each sample used for BN–PAGE and western blot represent a different myoblast line. Statistical analysis conducted was two-way ANOVA (a) and two-sided Student’s t-test (d,f).
