Extended Data Fig. 7: Neuronal changes after fasting.
From: HypoMap—a unified single-cell gene expression atlas of the murine hypothalamus
a, there is no change in Fos expression in Campbell et al. AgRP neurons after fasting. Left: UMAP plot depicting C66-46: Agrp.GABA-4 in HypoMap. The inset shows Agrp expression in Campbell et al. cells from C66-46: Agrp.GABA-4C66-46: Agrp.GABA-4. Right: UMAP plots of the same cells showing Fos expression in fasted and ad libitum fed states. b, CREB1 transcription factor binding site (TFBS) detection in IEG promoters compared to 1000 randomly sampled background gene promoters and IEG promoters. 1700016P03Rik contains multiple putative CREB1 binding sites in its promoter sequence, indicating it could play a role as an immediate early gene. c, Number of differentially expressed genes (DEG) after fasting in each cluster shown on the UMAP of nucSeq data. d, Enrichment of DEGs that were found in at least 20% of all clusters (155 genes) in ‘biological process’ gene ontology (GO) terms. The pvalues are based on a hypergeometric test from an over representation analysis and corrected using false discovery rate (fdr). Globally regulated genes are enriched in translation related terms and ‘cell death’.
