Extended Data Fig. 8: Physical and functional coupling of ADRA1A to Gαq.
From: ADRA1A–Gαq signalling potentiates adipocyte thermogenesis through CKB and TNAP
a-d, agonist-induced BRET between ADRA1A-tagged Nano Luciferase (ADRA1A-Nluc) and Venus-tagged (a) miniGαs, (b) miniGαi, (c) miniGαo, and (d) miniGα12 protein sensors in immortalized brown adipocytes (n = 3 per group). e, Basal oxygen consumption rates (related to experiments in Fig. 3d, e) of freshly isolated brown adipocytes treated with YM-254890 compared to vehicle (n = 5 per group). f, Catalytic properties of recombinant TNAP (n = 3 per group). Data are presented as mean ± s.e.m. and n numbers are of biologically independent experiments. e, two-tailed student’s t-test.
