Extended Data Fig. 9: Energy expenditure by Gαs and Gαq signaling in vivo.
From: ADRA1A–Gαq signalling potentiates adipocyte thermogenesis through CKB and TNAP
a, Western blot of BAT, SAT, PgAT, liver (Liv) and kidney (Kid) (n = 2 per group). b, RT-qPCR from BAT (hM3DqAdipoqCre:Ckbfl/+: n = 17, 9 males, 8 females) (hM3DqAdipoqCre:Ckbfl/fl: n = 14, 6 males, 8 females). c, RT-qPCR from BAT (n = 16: 8 males, 8 females per genotype). d, EE of hM3Dq:Ckbfl/+ or AdipoqCre:Ckbfl/+ mice injected i.p. with saline (n = 12: 8 males, 4 females for both groups). e, EE of hM3DqAdipoqCre:Ckb+/+ (n = 14: 8 males, 6 females) and hM3DqAdipoqCre:Ckbfl/fl (n = 16: 8 males, 8 females) following i.p. injection of CL (0.5 mg kg−1). f, EE of hM3Dq:Ckbfl/+ or AdipoqCre:Ckbfl/+ mice injected i.p. with CL or CL + DCZ (n = 12: 8 males, 4 females for both groups). g, EE of hM3DqAdipoqCre:Ckbfl/+ male mice in response to saline (n = 16), CL (n = 8), DCZ (0.5 mg kg−1) (n = 8), or DCZ (1 mg kg−1) (n = 8). h-j, Baseline (h) lean mass, (i) fat mass, and (j) body mass of hM3DqAdipoqCre:Ckb+/+ mice (CL: n = 13: 8 males, 5 females; CL + DCZ: n = 12: 8 males, 3 females). k-m, Baseline (k) lean mass, (l) fat mass, and (m) body mass of hM3DqAdipoqCre:Ckbfl/fl mice (CL: n = 14: 7 males, 7 females; CL + DCZ: n = 14: 7 males, 7 females). n-p, Baseline (n) lean mass, (o) fat mass, and (p) body mass of hM3Dq:Ckbfl/+ or AdipoqCre:Ckbfl/+ mice (n = 8 per group: 4 males, 4 females). q-s, movement of (q) hM3DqAdipoqCre:Ckb+/+ mice (CL: n = 13: 8 males, 5 females; CL + DCZ: n = 11: 8 males, 3 females), (r) hM3DqAdipoqCre:Ckbfl/fl mice (CL: n = 14: 7 males, 7 females; CL + DCZ: n = 14: 7 males, 7 females), and (s) control (hM3Dq:Ckbfl/+ or AdipoqCre:Ckbfl/+) mice (n = 8 per group: 4 males, 4 females). Data are presented as mean ± s.e.m. and n numbers are of biologically independent experiments. b, c, h-p two-tailed student’s t-test; d-f, q-s, Two-way ANOVA (Fisher’s LSD).
