Fig. 3: Thermogenesis by combined Gαq and Gαs signalling genetically requires Ckb in vivo.
From: ADRA1A–Gαq signalling potentiates adipocyte thermogenesis through CKB and TNAP
a, Schematic of BRET assay. b, Agonist-induced BRET in immortalized brown adipocytes (n = 3 per group). c, Cartoon depicting inhibition of Gαq signalling by YM-254890. d, Representative basal and NA-stimulated (0.1 μM) oxygen consumption trace of freshly isolated brown adipocytes, treated with YM-254890 (10 μM) or vehicle. The time of NA addition (arrow) was normalized to 100%. e, NA-stimulated oxygen consumption rates (above basal) of freshly isolated brown adipocytes (n = 5 per group). f, Cartoon of hM3DqAdipoqCre mouse construction. g, Schematic of activation of Gαs and Gαq signalling in adipocytes from hM3DqAdipoqCre mice. h,i, Energy expenditure (EE) with saline injection of hM3DqAdipoqCre:Ckbfl/+ mice (CL group, n = 6 males, 4 females; CL + DCZ group, n = 8 males, 4 females) (h) or hM3DqAdipoqCre:Ckbfl/fl mice (CL group, n = 11 males, 7 females; CL + DCZ group, n = 10 males, 7 females) (i). j,k, EE with drug injection of hM3DqAdipoqCre:Ckbfl/+ mice (CL, n = 6 males, 4 females; CL + DCZ, n = 8 males, 4 females) (j) or hM3DqAdipoqCre:Ckbfl/fl mice (CL, n = 11 males, 7 females; CL + DCZ, n = 10 males, 7 females) (k). l–n, Mean EE (l), daily EE (m) and cumulative EE (n) of hM3DqAdipoqCre:Ckb+/+ mice (CL, n = 8 males, 5 females; CL + DCZ, n = 8 males, 3 females). o–q, Mean EE (o), daily EE (p) and cumulative EE (q) of hM3DqAdipoqCre:Ckbfl/fl mice (CL, n = 7 males, 7 females; CL + DCZ, n = 7 males, 7 females). r–t, Mean EE (r), daily EE (s) and cumulative EE (t) of control (hM3Dq:Ckbfl/+ or AdipoqCre:Ckbfl/+) mice (n = 4 males, 4 females). Data are presented as the mean ± s.e.m. and n indicates the number of biologically independent experiments. e, two-tailed student’s t-test; h–m,o,p,r,s, two-way ANOVA (Fisher’s LSD); n,q,t, analysis of covariance (ANCOVA; two-sided, Bonferroni).
