Extended Data Fig. 8: Shift in balance of AgRP and Pomc gene expression in the hypothalamus of mice stimulated with food odor during fasting.
From: Food odor perception promotes systemic lipid utilization
C57BL/6 J mice were fasted for 24 h, and stimulated with or without NCD odor for 1 h in the SOS1 cage. The expression levels of AgRP and Pomc mRNAs in the hypothalamus were measured by RT-PCR. a, Relative expression levels of AgRP and Pomc mRNAs compared to 18 S rRNA. b, The AgRP/Pomc expression ratio. c, The mRNA levels of hypothalamic neuropeptides, Orexin (Hypocretin), melanin-concentrating hormone (Mch), and thyrotropin-releasing hormone (Trh), compared to 18 S rRNA. n = 12 biological replicates. *P < 0.05, **P < 0.01 determined by unpaired two-tailed t-test. Data are presented as mean ± s.e.m.
