Fig. 1: Obesity induces organ-specific changes in ECs.
a, Experimental design; n = 3 animals per group. The FACS plot shows exemplary gating for CD31+CD45low cells; sc, subcutaneous; vis, visceral; mo., months. b, Uniform manifold approximation and projection (UMAP) clustering of ECs from seven organs of mice on a WD or chow diet after filtering. Colors correspond to the organ from which the ECs were derived. Each dot represents a single EC; n = 3 animals per diet. c, Number of ECs analyzed from each organ after filtering out low-quality cells and non-ECs. d, Schematics of major vessel types. e, Number of DEGs (adjusted P value of <0.05 and | log (fold change (FC)) | > 0.1) in ECs from the different organs of obese versus control mice in (1) art, (2) cap and (3) ven. f, Correlation of gene expression changes in obese versus control conditions across art, cap and ven ECs. Genes showing a | log (FC) | > 0.1 in any tissue for the indicated EC population were used to collate the list of genes used for these analyses. A Pearson r value for each comparison is provided. Adjusted P value indicates adjustments for multiple comparisons using the Benjamini–Hochberg method (e).
