Extended Data Fig. 5: Itaconate impedes aspartate and serine/glycine biosynthesis in CD8 + T cells.
From: Myeloid-derived itaconate suppresses cytotoxic CD8+ T cells and promotes tumour growth
(A) Levels of various glycolytic intermediates in CD8 + T cells exposed to exogenous itaconate (ITA) or NaCl (Ctrl), as revealed by metabolite profiling analyses (n = 3 biologically independent samples). (B) Levels of various nucleotides in CD8 + T cells exposed to exogenous itaconate or NaCl (Ctrl), as revealed by metabolite profiling analyses (n = 3 biologically independent samples). (C) Incorporation of 13 C to Aspartate from [U-13C]-glucose in CD8 + T cells exposed to exogenous itaconate or NaCl (Ctrl) (n = 4 biologically independent samples). (D) Effects of nucleosides supplementation on the proliferation of mouse CD8 + T cells treated with 3 mM exogenous itaconate, as measured by CFSE dilution (n = 3 biologically independent experiments). (E) Representative CFSE intensity histograms from flow cytometry analysis for (D). (F) Effects of aspartate (Asp) and serine (Ser) supplementation on the ability of itaconate (3 mM) treated mouse CD8 + T cells on killing MC38-OVA cells (n = 5 biologically independent experiments). For all panels, p values were calculated by paired two- tailed t-tests. Data are mean ± SEM.
