Extended Data Fig. 3: Generation and validation of AgRP-Grb10-OE mice.
From: Hypothalamic Grb10 enhances leptin signalling and promotes weight loss
a. Schematic diagram of virus injection into the ARH. b. Representative GFP expression in the ARH of AgRP-IRES-Cre/Rosa26-LSL-tdTomato mice. c. Quantification for the percentage of GFP cells that are tdTomato+ (n = 3). d. Grb10 immunofluorescence staining in the ARH with one side of the AgRP neurons infected with AAV-DIO-GFP and the other side infected with AAV-DIO-Grb10-GFP. e. Representative immunofluorescence images of GFP and Grb10 expression in the ARH. f. Quantification for the percentage of GFP+ AgRP neurons (n = 5). g-h. Body weight (g) and percentage of mass weight (h) in female control (n = 7) and AgRP-Grb10-OE (n = 8) mice. i-j. Area under curve (AUC) for GTT (i) and ITT (j) in female control (n = 7) and AgRP-Grb10-OE (n = 8) mice. Data are expressed as mean ± SEM. Two-way ANOVA with Sidak’s or Tukey’s test (g, h) or two-tailed Student’s t test (i, j). *p < 0.05.
