Extended Data Fig. 9: Overexpression of Grb10 in POMC neurons has no effect on body weight and food intake under chow-fed condition.
From: Hypothalamic Grb10 enhances leptin signalling and promotes weight loss
a. Schematic diagram of virus injection into the ARH. b. Beta-endorphin immunofluorescence staining in the ARH with POMC neurons infected with AAV-DIO-Grb10-GFP. c. Quantification for the percentage of beta-endorphin+ GFP cells (n = 3). d. Representative Grb10 immunofluorescence staining in the ARH with one side of the POMC neurons infected with AAV-DIO-GFP and the other side infected with AAV-DIO-Grb10-GFP (n = 4). e. Representative immunofluorescence images of GFP and Grb10 expression in the ARH. f. Quantification for the percentage of GFP+ POMC neurons (n = 5). g-i. Body weight (g), cumulative food intake (h) and body composition (i) of chow-fed male control (n = 6) and POMC-Grb10-OE (n = 7) mice. Data are expressed as mean ± SEM. Two-way ANOVA with Sidak’s or Tukey’s test (g-i).
