Extended Data Fig. 3: Characterization of Hk2^CreERT2-tdTomato mice.

(a) Flow cytometry analyses of tdT⁺ cells and CD11b⁺CD45^low microglia in Hk2^CreERT2-tdTomato mice. (b) Representative micrographs (from 3 independent experiments) showing the co-labeling of tdT⁺, Iba1⁺and Hk2⁺ in brain slices from Hk2^CreERT2-tdTomato mice. Dotted boxes show regions of higher magnification. (c) FISH of Hk2 and tdT staining in brain slices from Hk2^CreERT2-tdTomato mice and quantification. Dotted boxes show regions of higher magnification. n = 3 mice per group. (d) Representative micrographs (from 3 independent experiments) showing the three-dimension (3D) reconstruction of a tdT⁺ cell in brain slices from Hk2^CreERT2-tdTomato mice using Imaris. (e) Representative micrographs (from 3 independent experiments) of tdT and different cellular markers, NeuN (neurons), Sox10 (oligodendrocytes) and GFAP (astrocytes) in the brains of Hk2^CreERT2-tdTomato mice. (f) Representative micrographs (from 3 independent experiments) showing full co-localization of GFP, tdT and Iba1 in brain slices from Hk2^CreERT2-tdTomato::Ai3^fl/+ mice after tamoxifen induction. TAM, tamoxifen; D, day; tdT, tdTomato. Data are presented as means ± SEM.