Extended Data Fig. 7: LDHA and Rac1 small-molecule inhibitors display a much more pronounced inhibitory effect on colony formation, migration and invasion of breast cancer cells.
From: Metabolic enzyme LDHA activates Rac1 GTPase as a noncanonical mechanism to promote cancer
a, The combination treatment displayed a much more pronounced inhibitory effect on colony formation of breast cancer cells. BT-549, MCF7 and ZR-75-1 cells were treated with indicated concentrations of FX11 and/or NSC23766 for 4 days before colony formation assays. Combo: FX11 + NSC23766. b, c, The combination treatment displayed a much more pronounced inhibitory effect on migration (b) and invasion (c) of breast cancer cells than the single inhibitor treatment as analyzed by Transwell assays. In b, c, BT-549, MCF7 and ZR-75-1 cells were treated with the indicated concentrations of FX11 and/or NSC23766 for 24 h. NSC: NSC23766. Data represent mean ± s.d. (n = 6 independent experiments), one-way ANOVA followed by Tukey’s test. *: P < 0.0001.
