Extended Data Fig. 9: The effect of FX11 and/or NSC23766 on the growth and lung metastasis of mammary tumors in MMTV-PyMT mice.
From: Metabolic enzyme LDHA activates Rac1 GTPase as a noncanonical mechanism to promote cancer
a, The effects of FX11 and/or NSC23766 on the growth and metastasis of mammary tumors in 10-week-old MMTV-PyMT mice. The 10-week-old female MMTV-PyMT mice that developed late-stage tumors were treated with FX11 (1 mg/kg/day; i.p.; once/day) and/or NSC23766 (1.5 mg/kg/day, i.p.; once/day) for 3 weeks before they were sacrificed for analysis. Data represent mean ± s.d.; n=6 mice/group. One-way ANOVA followed by Student’s t-test. Con: vehicle; NSC: NSC23766; combo: FX11 + NSC. b, The interaction between endogenous LDHA and Rac1 in both non-tumor and tumor mammary tissues of MMTV-PyMT mice detected by co-IP and western-blot assays. Non-tumor mammary tissues were obtained from 5-week-old MMTV-PyMT mice and mammary tumor tissues were obtained from 10-week-old MMTV-PyMT mice. Normal mammary tissues from 5-week-old LDHA-deficient mice (the R26-Cre-ERT2, LDHAflox/flox mice treated with Tamoxifen to delete LDHA as presented in Fig. 2h) were used as negative controls for assays. NC: negative control.
