Extended Data Fig. 7: Down-regulation of IsletMICs in T2D islets is not associated with changes in the expression of SRRM3 isoforms nor in co-regulatory RNA-binding proteins.
From: Pancreatic microexons regulate islet function and glucose homeostasis
(A, B) Expression of SRRM3 alternative isoforms containing the enhancer of microexons domain (eMIC; ENST00000611745) and with a truncated eMIC (ENST00000612155) in islets from normo-glycemic (ND), and type 2 diabetic individuals (T2D) from the studies of Wigger et al. 2021 (A) (n = 44 biologically independent samples) and Fadista et al. 2014 (B) (n = 40 biologically independent samples). (C, D) RNA expression of RNA-binding proteins reported to co-regulate microexon splicing in neurons in islets from normo-glycemic (ND), glucose intolerant (IGT) and type 2 diabetic individuals (T2D) from Wigger et al. 2021 (C) (n = 78 biologically independent samples) and Fadista et al. 2014 (D) (n = 54 biologically independent samples) studies. (E) RT-PCR analysis of microexon inclusion/exclusion after transfection with control or SRSF6 siRNAs in EndoC-βH1 beta cells. Data in (A–D) are shown as box-and-whisker plot, in which the lower and upper bounds of the box represent the upper and lower quartiles, the centre line represents the median and whiskers the 1.5x interquartile range. P values were obtained from two-sided Wilcoxon test.
