Extended Data Fig. 6: Verification of cell cycle synchronization of G-Loop1 CAD mutant cells and estimation of their CAD activity during the cell cycle.
From: Allosteric regulation of CAD modulates de novo pyrimidine synthesis during the cell cycle
a,b, Cell parameter measurement (a) and EdU incorporation (b) of wild-type HeLa cells synchronized by NOC. c,d, Cell parameter measurement (c) and (d) EdU incorporation (d) of wild-type HeLa cells synchronized by DTB. e, Estimation of CAD activity of wild-type HeLa cells synchronized by DTB. f,g, Cell parameter measurement (f) and EdU incorporation (g) of G-Loop1 HeLa cells synchronized by NOC. h,i, Cell parameter measurement (h) and EdU incorporation (i) of G-Loop1 HeLa cells synchronized by DTB. j, Estimation of CAD activity of G-Loop1 HeLa cells synchronized by DTB. Data are the mean ± s.d. (n = 3, biological replicates). Two-tailed Student’s t-test. *P < 0.1, **P < 0.01, ***P < 0.001, ****P< 0.0001, or NS.
